FISHView

What is FISH?

The fluorescent in situ hybridization technique (FISH), or chromosome painting, is used for the chromosomal location of a specific DNA (or RNA) probe. First, the probe is labeled with a fluorescent dye. It is then added to a chromosomal preparation and after annealing occurs, the chromosomes are viewed under a fluorescent microscope. The probe hybridizes to the chromosome carrying the sequence of interest. If the species has been characterized cytogenetically, the marker can be assigned to the appropriate chromosome.

FISHView

FISHView® is ASI's fully-automated image acquisition and analysis system for FISH. FISHView is one of ASI's CytoLabView modules for Cytogenetics. Other related products for FISH are: Manual Counting, SpotCView, MultiStain and SpotScan.

Acquisition (Image Capture)

The FISHView capture process is quick and simple with minimal human intervention. It incorporates powerful manual and automatic control over contrast, brightness, and sharpness levels of the entire image and individual signals. Automatic background correction compensates for nonconformities of the sample and microscope.

FISHView is compatible with any fluorescence microscope and can be integrated with a monochrome, color or cooled digital high resolution camera. The 12 bit digital camera with its large (2/3”) field of view captures with the highest quality possible in a single-second operation.

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Analysis Features

FISHView analysis performs automatic and manual segmentation to obtain the morphology and intensity of each object, complete with its color layers. Dedicated algorithms for detection and quantification of multiple small signals, such as telomeric probes performing 3D image data, are easily exported to popular analysis and visualization software programs. Z-stacking is simple and accurate, even on manual microscopes , for viewing all signals in focus.

FISHView supports full karyotyping.