HiSKY

About Spectral Karyotyping (SKY®)

The SKY method involves several steps. First, each chromosome is labeled with a different combination of fluorescent dyes.  The spectral imaging system then measures the full visible light spectrum emitted from the sample to acquire a spectral image of the chromosomes.  HiSKY, the analysis software, analyzes the results of the spectral image and displays the image so that chromosomal abnormalities are easily identified.  The end result is a metaphase and karyotype image where each pair of chromosomes appears with a distinct color.  This allows easy identification of translocations and components of marker chromosomes.

HiSKY, the supreme multicolor FISH application available in today's market, incorporates user friendliness and simplicity with many popular tools and improvements to SKY.  These advanced features include a list of new user interface elements, advanced algorithms and improved capabilities, allowing easier work that brings faster and more informative results.  Users new to multicolor FISH will find HiSKY a must to add to their arsenal of research and diagnostic tools.  Hundreds of professional articles attest to the HiSky's proven success in attaining definitive results.  To read sample articles, click here.

Key Features 

• One button capture
• No need for classifier training
• Automatic, robust color karyotyping of all sample types using a spectral imaging system
• Automatic, accurate identification of translocations and chromosomal origins of marker chromosomes
• Tool tip with exact color content percentage
• Special tool to identify small translocations and insertions
• Highest quality band image enhancement
• Powerful spectral image analysis tools
• Easy annotation and report tools 

HiSKY Interface

There are five windows in the HISKY interface: Spectral Color Image, Zoom View, Band Image, Information and Karyotype Table.  Each window provides different information about the metaphase under analysis.

Spectral Color Image: displays a version of the current image with enhanced colors showing spectral differences
Band Image: displays the band image from a HiSkyTIFF, DIB or JPEG format project file
Classified Image: displays each image pixel according to its classification color. This option is available only after the image is karyotyped.

1. Zoom View window – active image under different levels of magnification
2. A Karyotype table window
3. Information window: the Information window gives you spectral information about the following items: current cursor coordinates, spectrum at the current cursor position, reference spectra after preprocessing, marked pixels you want to analyze, and intensity levels of the current pixel

For additional information on HiSKY's advanced color control, powerful analysis tools and more – click here

In the US, the HiSKY is not cleared for In Vitro Diagnostic procedures and is intended for research use only.