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SPOTScan
Automatic Spot Counting and Signal Evaluation
SpotScan is one of the applications running on Applied Spectral Imaging’s automatic scanning platform, ScanView. ScanView is created on an open and modular platform on which common hardware allows the use of different software modules for a variety of applications in genetics, hematology, pathology, and much more.
Spot counting, including generation of cell statistics is becoming a clinical need for new cancer genetics markers such as HER-2/neu. The regulations require quantiation of 60 to hundreds of cells to be used for a complete evaluation, which is an exhausting task for manual counting. An automated system that scans and quantitates multiple slides has a clear advantage in budgetary and manpower efficiency. SpotScan has been designed to bring both benefits of efficiency and accuracy to genetics and pathology laboratories. SpotScan is an ideal solution for multiple marker assessment or quality control protocols.
 SpotCScan-X,Y,18 |
SpotScan provides a modular approach to both work procedures and applications. From a stand-alone single system configuration used for a specific laboratory task to multiple-system server networks to accommodate the needs of multiple users, the SpotScan system will become central to your laboratories imaging and analysis needs.
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In its standard configuration, SpotScan scans up to eight slides in multiple color channels. It supports up to 6 filter cubes and controls an optional filter wheel to enable more than 6 colors. After a short slide setup process, SpotScan scans the counterstain layer and finds the correct focus map based on a sparse grid of positions within the scan area. Subsequently, the slide is scanned for each of the existing dyes. Multiple focal planes are acquired to extract focused Z-stack images, and the user may optionally save all 3D data, in individual layers, for review at a later time. Segmented cells are displayed in a gallery during capture, as well as a slide view with cell locations and statistics.
 Sample with All-telomer probe. SpotScan has the ability to count 500 and more signals per cell |
© 2004 Applied Spectral Imaging, all rights reserved. Revised
September 2004. www.spectral-imaging.com
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FAQ's
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